| 產(chǎn)品名稱 |
pRT432-1 |
| 商品貨號(hào) |
B225313 |
| Designations |
pRT432-1 |
| Species |
avian myeloblastosis virus (type C) |
| Depositors |
Molecular Genetics, Inc., SL Misrock, Molecular Genetics, Inc. |
| Applications |
produces protein reverse transcriptase, beta-subunit |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references: DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: avian myeloblastosis virus
Gene name: reverse transcriptase, beta-subunit
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb):
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: avian myeloblastosis virus
Gene name: reverse transcriptase, beta-subunit
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb):
Cross references: Gene product: reverse transcriptase, beta-subunit Target Gene: reverse transcriptase, beta-subunit |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.8, 3.3, 0.26; PstI--4.0, 3.2; BamHI--7.2. The insert contains the complete coding sequence for the beta subunit of the reverse transcriptase gene under the control of the lac promoter. |
| References |
Weis JH, Salstrom JS. Production of reverse transcriptase. US Patent 4,663,290 dated May 5 1987
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
