| Vector |
Construct size (kb): 4.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TPM*
Gene name: tropomyosin (non-muscle)
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: T05399; EST: EST03288; Primer: M13-21; THC
id: THC124136
DNA Seq. Acc.: X04588; EST: EST03288; Primer: M13-21
Nucleotides 1-256 of the insert correspond to
nucleotides 1534-1789 of X04588 with 97% identity. Insert lengths(kb): 1.399999976158142 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: tropomyosin (non-muscle) [TPM*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.0, 0.4. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. |
