| 產品名稱 |
pAQP1 Xenopus expression vector [UTR-01749, WI-9113, pCHIPev/BSII KS-] |
| 商品貨號 |
B225208 |
| Designations |
pAQP1 Xenopus expression vector [UTR-01749, WI-9113, pCHIPev/BSII KS-] |
| GenBank Number |
M77829
|
| Species |
Homo sapiens, human |
| Depositors |
P Agre, GM Preston |
| Applications |
construct to generate mRNA that can be expressed in Xenopus oocytes aquaporin 1 (channel-forming integral protein, 28 kD) |
| Vector |
Construct size (kb): 4.057000160217285 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: HindIII
Vector end: PstI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AQP1
Other gene symbols: CHIP28
Gene name: aquaporin 1 (channel-forming integral protein, 28 kD)
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 p14; 7, 84.8799999 cR
Tissue: bone marrow
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: PstI
Insert size (kb): 1.114
Cross references: DNA Seq. Acc.: M77829
Nucleotides 57-904 of the insert correspond to
nucleotides ~1-860 of M77829. Insert lengths(kb): 1.113999962806702 Tissue: bone marrow Gene product: aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1] Target Gene: aquaporin 1 (channel-forming integral protein, 28 kD) |
| Insert Size (kb) |
1.114 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.3, 1.1, 0.22; HindIII/PstI--2.8, 1.2; PvuII--2.4, 0.78, 0.6, 0.22. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.08, 0.27; PvuII--0.34, 0.53. The AQP1 coding sequence is flanked by 56 bp 5' untranslated sequence and 210 bp 3' untranslated sequence from the Xenopus beta-globin gene. Sense mRNA can be synthesized in vitro from the SmaI digested contruct using T3 RNA polymerase. Resulting mRNA can be injected into and expressed in Xenopus oocytes. |
| References |
Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319
Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524
Peter Agre, personal communication
|
