| 產品名稱 | L10BIOBR-GFP |
|---|---|
| 商品貨號 | B225042 |
| Organism | Mus musculus, mouse |
| Cell Type | melanocyte |
| Product Format | frozen |
| Morphology | melanocyte |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | newborn |
| Strain | B10.BR |
| Applications | Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC? CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma [PubMed: 12514183]. The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC? CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma [PubMed: 12514183]. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy. |
| Tumorigenic | No |
| Effects | No, progressive tumor growth was not observed in nude mice Ref   Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183 |
| Comments | The L10BIOBR-GFP cell line was derived as a negative control for CRL-2771. The immortalized murine melanocyte cell line L10BIOBR was transduced with pDIVA-GFP and subjected to puromycin selection. Together, L10BIOBR-GFP (CRL-2770) and L10BIOBR-MAPKK (ATCC? CRL-2771) are valuable cell models for oncogenic transformation and signal transduction studies for melanoma [PubMed: 12514183]. Please note, although the L10BIOBR-GFP cell line harbors the gfp gene, as verified by PCR analysis, the cell line does not express sufficient GFP protein for detection of GFP fluorescence by flow cytometry or fluorescence microscopy. |
| Complete Growth Medium | Ham's F10 medium supplemented with 50 ng/ml TPA (Sigma Catalogue No. P-8139) and 7% horse serum
|
| Subculturing | Protocol:
Interval: Subculture when cells reach a concentration of 2 X 10(4) cells/sq. cm. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time | 29 hours |
| Name of Depositor | JL Arbiser |
| Year of Origin | January 1, 2002 |
| References | Govindarajan B, et al. Malignant transformation of melanocytes to melanoma by constitutive activation of mitogen-activated protein kinase kinase (MAPKK) signaling. J. Biol. Chem. 278: 9790-9795, 2003. PubMed: 12514183 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
