| 產品名稱 |
pDR540 |
| 商品貨號 |
B224059 |
| Designations |
pDR540 |
| Depositors |
GN Bennett |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 0E-16 Vector: pDR540 (plasmid) Promoters: Promoter tac Construction: pKO1, Escherichia coli trp-lac fusion promoter tac Marker(s):ampR,galK+ Construct size (kb): 0.0 Features: marker(s): ampR, galK+
promoter: tac
replicon: pMB1 |
| Applications |
contains easily purifiable cassette(s) for construction |
| Comments |
This plasmid is unstable when transformed into C600 galK-. Contains an easily purifiable trp-lac hybrid promoter which can be used to construct expression vectors. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Russell DR, Bennett GN. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing. Gene 20: 231-243, 1982. PubMed: 6299890
|
| Shipped |
freeze-dried |
