| 產品名稱 |
pLamB2(21/1) |
| 商品貨號 |
B223286 |
| Designations |
pLamB2(21/1) |
| GenBank Number |
J02930
|
| Species |
Mus musculus, mouse |
| Depositors |
I Oberbaumer |
| Applications |
The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization. |
| Vector |
Construct size (kb): 5.199999809265137 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.517999887466431 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: laminin, gamma 1 (formerly LAMB2)(laminin, B2 polypeptide) [Lamb2] Alleles: a, b, d, d |
| Insert Size (kb) |
2.518 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.6 (doublet); EcoRI--5.2; HindIII--3.4, 1.8; XbaI--3.6, 1.6; PstI--4.2, 1.0. The insert contains the following restriction sites (approximate kb from the 5' end): StuI--0.02, 1.91; HindIII--0.78; XbaI--1.02; PstI--1.68; SacI--1.99; SspI--2.5. Extends from nt 5036 to 7544 of the reported sequence, containing most of the 3' untranslated region. The 1.9 kb StuI or 1.7 kb HindIII/SspI fragments are recommended for hybridization. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975
Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.
Durkin ME, et al. The primary structure of the mouse laminin B2 chain and comparison with laminin B1. Biochemistry 27: 5198-5204, 1988. PubMed: 3167041
Ilse Oberbaumer, personal communication
|
