ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites (nt position according to published sequence): SacII--68; PvuII--450; PstI--455, 1538; BamHI--556; BglII--1044, 1858, 1918; BstXI--1399.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--3.4, 1.7; HindIII--5.0; PstI--3.6, 1.1, 0.6; BglII--4.2, 0.9.
Contains the complete coding sequence (nt 2-1507).
Detects a mRNA of 2.4 kb.
Oriented such that transcription from the SP6 promoter gives the sense strand.
Buonanno A, et al. A universal oliglonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit. J. Biol. Chem. 261: 16451-16458, 1986. PubMed: 3782129
Heidmann O, et al. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science 234: 866-868, 1986. PubMed: 3022377
