| Vector |
Construct size (kb): 7.099
Name of vector: pcD
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: XhoI BamHI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
Enhancer: SV40
Marker(s): ampR
Promoter: SV40 early, late
Replicon: pMB1, SV40
Terminator: SV40 late |
| Insert |
DNA: cDNA
Insert Lengths (kb): 4.0
Tissue: gastric cancer MKN-7 cell line
Gene Product: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)( Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2, Neu oncogene, avian erythroblastosis oncogene B 2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogen, tyrosine kinase, receptor HER2) [NGL]
Genome: Homo sapiens
Gene Symbol: ERBB2
Other Gene Symbols: NGL
Genomic Copy Number: unique
Contains Complete Coding Sequence?: U
Chromosome: 17; Localization: 17 q11.2-q12
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 4.0
Cross References: DNA Seq. Acc.: X03363 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--7.1; PvuII--3.0, 2.0, 0.9, 0.5, 0.4, 0.3; BamHI--4.9, 1.3, 0.7, 0.2; EcoRI--3.8, 1.8, 1.5.
Restriction digests of the clone give the following sizes (kb): HindIII--6.8; PvuII--2.95, 1.95, 0.85, 0.5, 0.4, 0.25; HindIII/PvuII--2.3, 1.95, 0.85, 0.54, 0.5, 0.4, 0.25; BamHI--4.7, 1.15, 0.6, 0.2; EcoRI--3.7, 1.65, 1.35.
The insert is not readily excised from the vector (flanked by GC and AT tails). StuI cuts twice in the insert but not in the vector. The vector is cut with HindIII. A StuI/HindIII double digest gives a 3.4 kb fragment specific to c-erbB-2.
Contains AccI, BamHI, EcoRI, SmaI, PvuII, and KpnI sites.
Includes sequence from approximately 500 to end in Fig. 2 encoding amino acids from about 100-1255 (including the ATP binding site of the tyrosine kinase domain). |
| References |
Zhang X, et al. Amplification and rearrangement of c-erb B proto-oncogenes in cancer of human female genital tract. Oncogene 4: 985-989, 1989. PubMed: 2569708
Yamamoto T, et al. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor. Nature 319: 230-234, 1986. PubMed: 3003577
Fukushige S, et al. Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line. Mol. Cell. Biol. 6: 955-958, 1986. PubMed: 2430175
Ishii S, et al. Characterization of the promoter region of the human c-erbB-2 protooncogene. Proc. Natl. Acad. Sci. USA 84: 4374-4378, 1987. PubMed: 2885835
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