| 產品名稱 |
HFBCE57 |
| 商品貨號 |
B222657 |
| Designations |
HFBCE57 |
| GenBank Number |
M77954
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 3.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PDHA1
Gene name: pyruvate dehydrogenase, E1 alpha polypeptide 1
Contains complete coding sequence?: U
Chromosome: X; Localization: X p22.1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: M77954; EST: EST01538; Primer: M13 Forward;
THC id: THC111996 Insert lengths(kb): 0.8999999761581421 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: pyruvate dehydrogenase, E1 alpha polypeptide 1(pyruvate dehydrogenase E1-alpha subunit) [PDHA1] |
| Insert Size (kb) |
0.900 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.9; BamHI--3.9; PstI--3.6, 0.3. EST01538 matches GenBank accession HUMPDHA with 99.68% identity over the sequence range: 1-323. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
