Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--3.2, 2.9; BamHI--4.1, 2.0; EcoRI--3.5, 1.9, 0.65; PstI--3.3, 2.8; SacI--3.6, 2.6. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.12; EcoRI--1.82; BamHI--1.97. The 3' XbaI site is not cleaved by XbaI. Sequence analysis indicates that the site is present. Corresponds to nt 87-3039 of the sequence record. Contains the complete coding sequence (nt 190-2451 of the sequence record). |
