Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; HindIII--4.5; NdeI--4.5; PstI--4.3, 0.21. DNA containing this sequence is underrepresented in most genomic libraries because most restriction enzymes (except HinfI and TaqI) generate fragments with pHuR98 elements larger than 20 kb. The repeat sequence of this member of the satellite 3 family consists of GAACCCGAGT followed by diverged GGAAT repeats. This insert contains repeat spacing of 54 and 45 nt, separated by a 29 nt repeat. |
