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Human adenovirus 3

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編號:B221462

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產品名稱	Human adenovirus 3
商品貨號	B221462
Classification	Adenoviridae, Mastadenovirus, Human adenovirus B
Deposited As	Adenovirus type 3
Agent	Human adenovirus 3
Strain	GB
Applications	Respiratory research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen 1 mL per vial
Storage Conditions	-70°C or colder
Comments	Agglutinates rhesus monkey red blood cells. Weakly oncogenic, contains soluble group-specific CF antigen.
Effect on Host	CPE, rounding, clumping, and sloughing
Recommended Host	A549 (ATCC^® CCL-185™)
Growth Conditions	Temperature: 37°C Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-3 days
Name of Depositor	RJ Huebner, National Institute of Allergy and Infectious Diseases
Source	Nasal washings from an adult male with a common cold, Maryland, 1953
References	Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

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