Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 4.2; HindIII--4.8, 2.8; BamHI--8.3. A 2.9 kb EcoRI/HindIII subfragment detects a PvuII RFLP. Insert includes 2.4 kb of unique sequence and a 1.5 kb HindIII/EcoRI fragment containing repetitive sequence. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The PvuII polymorphism has demonstrated co-dominant segregation in 2 families. Only one allele is present in mouse/human hybrids with one chromosome 17. This is a subclone of lambdahe-A1 (p01239). Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HinfI, KpnI, PstI, TaqI. |
