The suggested host for vector amplification is E. coli C600 (ATCC 23724) and for library amplification is E. coli JC8679 (ATCC 47001). Vector for efficient transduction of mammalian cells with a cDNA library constructed with the pcD (Okayama and Berg) expression vector. Cloning capacity: 12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector. The order of the major features in this bacteriophage is: lambda762/deltab - HindIII - 0.5 kb SV40 fragment, map units 0.86-0.95 - reddelta - SalI - SV40 ori - SV40 early promoter - SV40 late 19S and 16S splice junctions - neo (Tn5) - SV40 late poly(A) - pMB1 ori - ampR - lambda762/KH54/lambda ori/nin5/QSR. |
