Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; EcoRI/HindIII--2.9, 1.75; HindIII--4.8; BamHI--uncut; XbaI--uncut. Insert contains sequence from the 5th intron of the argininosuccinate synthetase gene. The population studied was CEPH (Centre d'Etude du Polymorphisme Humain) families. Enzyme(s) not detecting polymorphism: BamHI EcoRV KpnI ScaI SstI BanI BclI BglI BglII EcoRI HincII BanII HinfI MboI MspI RsaI TaqI HindIII PssI PstI PvuII and XbaI. |
