| 產(chǎn)品名稱 | 1G12 |
|---|---|
| 商品貨號 | B218880 |
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type | hybridoma: B lymphocyte; somatic cell hybri |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain | CB.17 |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes the 3A9 T cell receptor (TCR). |
| Comments | Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody recognizes the 3A9 T cell receptor (TCR). |
| Complete Growth Medium | Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
|
| Subculturing | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 8 X 10(4) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype | IgG1 kappa |
| Population Doubling Time | 10 hours |
| Name of Depositor | ER Unanue |
| Year of Origin | 1996 |
| References | Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007 Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
