Restriction digests of the clone give the following sizes (kb): BglI--2.2, 1.6; PvuI--2.1, 1.8; PstI--3.9 One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The PstI restriction site from the URA3 gene of S. cerevisiae was removed by using oligo-directed mutagenesis. |
