| 產品名稱 |
human Id-1b [phId-1b] |
| 商品貨號 |
B218482 |
| Designations |
human Id-1b [phId-1b] |
| GenBank Number |
S78825
|
| Species |
Homo sapiens, human |
| Applications |
contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1 |
| Vector |
Construct size (kb): 4.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ID1
Gene name: inhibitor of DNA binding 1, dominant negative
helix-loop-helix protein
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q11
Tissue: U251 glioblastoma cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.145
Cross references: DNA Seq. Acc.: S78825 Insert lengths(kb): 1.144999980926514 Tissue: U251 glioblastoma cell line Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) [ID1] Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein |
| Insert Size (kb) |
1.145 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; BglI--2.2, 1.3, 0.7; KpnI--3.5, 0.65; PvuI--2.1, 1.1, 1.0, 0.1; PvuII--2.5, 1.4, 0.3. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.42; KpnI--0.54; PvuI--0.70, 0.83; PvuII--0.05; SmaI--0.36; SphI--0.70. There is an erroneous single extra nucleotide "t" at position 1091 in GenBank record S78225. The clones human Id-1a (ATCC 99522/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene. |
| References |
Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581
Mark A Israel, personal communication
|
