| Vector |
Construct size (kb): 6.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBC/CMV
Intact vector size: 4.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII
Host range: eukaryotic cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1
promoter for expression: CMV, ->
other: rat preproinsulin II (rI2) intron, ->
terminator: SV40 polyadenylation signal, ->
marker(s): ampR
replicon: SV40
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HAO-PEN
Gene name: 3-hydroxyanthranilate 3,4-dioxygenase
Contains complete coding sequence?: Y
Tissue: hepatoma HepG2 cell line (ATCC HB8065)
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.276
Cross references: DNA Seq. Acc.: Z29481
Nucleotides 1-1247 of the insert correspond to
nucleotides 1-1247 of Z29481. Insert lengths(kb): 1.276000022888184 Tissue: hepatoma HepG2 cell line (ATCC HB8065) Gene product: 3-hydroxyanthranilate 3,4-dioxygenase [HAO-PEN] Target Gene: 3-hydroxyanthranilate 3,4-dioxygenase |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 1.3; NcoI--4.0, 0.9, 0.5, 0.4, 0.3; PstI--5.4, 0.6. Insert contains the following restriction sites (approximate kb from the 5' end): NcoI--0.41, 0.91; PstI--0.62, 1.18; PvuII--0.21, 0.53, 0.73; SmaI--0.03; StuI--0.70; XhoI--0.95. Includes 40 nt of 5' untranslated sequence, the complete coding sequence and 346 nt of 3' untranslated sequence followed by a poly(A) tail |
