| 產品名稱 |
N1.551 |
| 商品貨號 |
B165216 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the fast IIa isoform of rodent skeletal muscle myosin. It cross-reacts with the IIx isoform, and does not react with the IIb isoform. |
| Derivation |
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rodent fast IIa myosin heavy chain (MyHC) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rodent fast IIa myosin heavy chain (MyHC) |
| Comments |
Animals were immunized with partially purified human neonatal (5 days postpartum) skeletal muscle myosin heavy chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with the fast IIa isoform of rodent skeletal muscle myosin. It cross-reacts with the IIx isoform, and does not react with the IIb isoform. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml 10 to 15 minutes, the flask may need to be shaken firmly to dislodge the cells). Add fresh medium, aspirate and dispense into new flasks. |
| Isotype |
IgM |
| Name of Depositor |
HM Blau |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Condon K, et al. Development of muscle fiber types in prenatal rat hindlimb. Dev. Biol. 138: 256-274, 1990. PubMed: 2108065
|
