| 產品名稱 |
MECA-89 |
| 商品貨號 |
B165141 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1) |
| Comments |
Animals were immunized with endothelial cells from mouse mesenteric and peripheral lymph nodes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer). Unlike MECA-367, MECA-89 does not inhibit alpha 4, beta 7 dependent binding in vitro. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 with 4.5 g/L glucose, 15 mM HEPES, 2 mM L-glutamine,
0.01 mM non-essential amino acids, and 0.05 mM 2-mercaptoethanol, 90%; fetal
bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
EC Butcher, DP Andrew |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147
Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297
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