| 產品名稱 |
ME361-S2a [ME361S2a] |
| 商品貨號 |
B165138 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with GD2 and GD3 gangliosides. It reacts preferentially with the GD2 and is largely unreactive with other gangliosides. It may be useful for diagnosis and therapy of human melanoma. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Clinical Data |
It may be useful for diagnosis and therapy of human melanoma. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against GD2 and GD3 gangliosides |
| Cellular Products |
immunoglobulin; monoclonal antibody; against GD2 and GD3 gangliosides |
| Comments |
Animals were immunized with the SK MEL 23 human melanoma cells line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with GD2 and GD3 gangliosides. It reacts preferentially with the GD2 and is largely unreactive with other gangliosides. It may be useful for diagnosis and therapy of human melanoma. This line is an isotype switch variant of ME361 (see ATCC HB-9325). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
Wistar Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Thurin J, et al. Monoclonal antibodies against melanoma-associated antigens and hybrid cell lines producing these antibodies. US Patent 4,849,509 dated Jul 18 1989
Thurin J, et al. GD2 ganglioside biosynthesis is a distinct biochemical event in human melanoma tumor progression. FEBS Lett. 208: 17-22, 1986. PubMed: 3533633
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