| 產品名稱 |
MC-IXC |
| 商品貨號 |
B165114 |
| Organism |
Homo sapiens, human |
| Tissue |
brain; derived from metastatic site: supra-orbital area |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
neuroblastoma |
| Age |
14 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
doubles minutes (DM) are prevalent |
| Derivation |
MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass. |
| Clinical Data |
14 years Caucasian female |
| Antigen Expression |
Blood Type O; Rh+ |
| Comments |
MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.
The choline acetyltransferase activity of MC-IXC was approximately four times higher than the parental line but MC-IXC was negative for dopamine beta hydroxylase activity as was the parental line.
MC-IXC cells have a reported saturation density greater than 1 X 106 cells/cm2.
The cells are fibroblast-like and grow to form a dense monolayer.
Floating cells are nonviable. |
| Complete Growth Medium |
The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C
Subculture Ratio: 1:10 to 1:20
Medium Renewal: Every 4 to 7 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 11 D7S820: 8 THO1: 9.3 TPOX: 9,11 vWA: 17,18 |
| Population Doubling Time |
36 hrs |
| Name of Depositor |
JL Biedler |
| Deposited As |
Homo sapiens |
| Year of Origin |
1971 |
| References |
Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425
Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704
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