| 產(chǎn)品名稱 |
M2-10B4 |
| 商品貨號(hào) |
B165048 |
| Organism |
Mus musculus, mouse |
| Tissue |
bone marrow/stroma |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a (C57BL/6J X C3H/HeJ)F1 mouse. |
| Genes Expressed |
laminin; collagen IV |
| Cellular Products |
laminin; collagen IV |
| Comments |
The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a (C57BL/6J X C3H/HeJ)F1 mouse. The cells express laminin and collagen IV, but do not express collagen I or Factor VIII. The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
CJ Eaves |
| Deposited As |
Mus musculus |
| References |
Sutherland HJ, et al. Differential regulation of primitive human hematopoietic cells in long- term cultures maintained on genetically engineered murine stromal cells. Blood 78: 666-672, 1991. PubMed: 1713512
Lemoine FM, et al. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp. Hematol. 16: 718-726, 1988. PubMed: 3261251
Lemoine FM, et al. Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Cancer Res. 48: 6438-6443, 1988. PubMed: 3263188
Lemoine FM, et al. Transformation-associated alterations in interactions between pre-B cells and fibronectin. Blood 76: 2311-2320, 1990. PubMed: 2147863
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