| 產品名稱 |
M17/4.4.11.9 (new clone of M17/4.2) |
| 商品貨號 |
B165042 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells. The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the alpha subunit of LFA-1 (mouse cytotoxic T cell antigen, CD11a) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the alpha subunit of LFA-1 (mouse cytotoxic T cell antigen, CD11a) |
| Comments |
Animals were immunized with cytotoxic T cells from C57BL/6 mice immunized with P815 cells. Spleen cells were fused with NS-1 myeloma cells. LFA-1 is a cell surface antigen found on cytolytic T lymphocytes. Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells. The LFA-1 and Mac-1 antigens are composed of two chains or subunits, alpha and beta. The beta chain of LFA-1 (Leukocyte Function Associated antigen) is immunologically identical to the beta chain of the Mac-1 antigen. The line was recloned by TA Springer in 1988, and a new seed stock was created at the ATCC. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Interval: Maintain between 5 X 10(4) and 5 X 10(5) cells/ml. Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
rat IgG2a kappa |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Sanchez-Madrid F, et al. Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and MAC-1. J. Exp. Med. 158: 586-602, 1983. PubMed: 6193226
Sanchez-Madrid F, et al. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73: 1-11, 1982. PubMed: 6983917
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