| Antigen Expression |
HLA-DR; Homo sapiens, expressed CD19; Homo sapiens, expressed CD10; Homo sapiens, expressed |
| Genes Expressed |
CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed |
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 105 viable cells/mL. Subculture when cell density reaches 1 x 106 cells/mL.
Medium Renewal: 2 to 3 times per week |
| References |
Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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