| 產品名稱 |
J1j.10 |
| 商品貨號 |
B164872 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Thy-1.2 antigen; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Thy-1.2 antigen; cytotoxic |
| Tumorigenic |
Yes |
| Effects |
Yes, in immunosuppressed (C57BL/B6 X CBA)F1 mice |
| Comments |
Animals were immunized with C3H/HeJ thymocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not lyse Thy-1.1 cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Modified Dulbecco's medium, 90%; horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
J Sprent |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Bruce J, et al. A monoclonal antibody discriminating between subsets of T and B cells. J. Immunol. 127: 2496-2501, 1981. PubMed: 6975326
|
