| 產(chǎn)品名稱 | IM-9 |
|---|---|
| 商品貨號 | B164858 |
| Organism | Homo sapiens, human |
| Tissue | peripheral blood |
| Cell Type | B lymphoblast; Epstein-Barr virus (EBV) transformed |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | multiple myeloma |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | normal human female; diploid; stable; Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation | Although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. |
| Clinical Data | Caucasian
female |
| Antigen Expression | CD11a +; CD19 +; CD20 +; CD38 -; CD49e + |
| Receptor Expression | growth hormone receptor
insulin receptor
calcitonin receptor
|
| Genes Expressed | immunoglobulin |
| Cellular Products | immunoglobulin |
| Comments | The cells are EBNA positive. ATCC confirmed this cell line is positive for the presence of Epstein-Barr (EBV) viral DNA sequences via PCR. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1x 105 and 1 x 106 viable cells/mL. Do not allow the cell density to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10,11 D13S317: 9,11 D16S539: 9,13 D5S818: 13 D7S820: 11,12 THO1: 6,9.3 TPOX: 11 vWA: 14,17 |
| Isoenzymes | ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 0 |
| Isotype | IgG kappa , IgG; kappa light chain
Ref  van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 |
| Name of Depositor | DN Buell |
| Deposited As | Homo sapiens |
| Year of Origin | 1967 |
| References | Fahey JL, et al. Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221-234, 1971. PubMed: 5290016 Lesniak MA, et al. Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J. Biol. Chem. 249: 1661-1667, 1974. PubMed: 4361817 Van Obberghen E, et al. Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J. Biol. Chem. 251: 6844-6851, 1976. PubMed: 185222 Gavin JR, et al. Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc. Natl. Acad. Sci. USA 71: 84-88, 1974. PubMed: 4359334 van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375 Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762 van Boxel JA, Buell DN. IgD on cell membranes of human lymphoid cell lines with multiple immunoglobulin classes. Nature 251: 443-444, 1974. PubMed: 4608784 |
| Cross References | Nucleotide (GenBank) : X81121 H.sapiens mRNA for central cannabinoid receptor, short isoform. Nucleotide (GenBank) : X84369 H.sapiens RNA for heavy chain variable and constant regions (IM9-VH). Nucleotide (GenBank) : U07989 Human Burkitt's lymphoma immunoglobulin kappa light chain mRNA, partial cds. Nucleotide (GenBank) : U07985 Human myeloma immunoglobulin heavy chain Fd region V-D-J-CH1 mRNA, partial cds. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
