| 產(chǎn)品名稱(chēng) |
IgG-IB7 |
| 商品貨號(hào) |
B164847 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the alpha subunit of farnesyltransferase in cultured mammalian cells. It is useful for studying the presence of the alpha subunit of farnesyltransferase in cell extraxcts. This antibody may be used as a control for the diagnostic test for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis (see ATCC CRL-2419). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the alpha subunit of farnesyltransferase |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the alpha subunit of farnesyltransferase |
| Comments |
Animals were immunized with a GST-farnesyltransferase fusion protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the alpha subunit of farnesyltransferase in cultured mammalian cells. It is useful for studying the presence of the alpha subunit of farnesyltransferase in cell extraxcts. This antibody may be used as a control for the diagnostic test for choroideremia (CHM), an X-linked form of retinal degeneration, by immunoblot analysis (see ATCC CRL-2419). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 104 and 5 x 105cells/mL. Do not allow the cell concentration to exceed 5 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases).
|
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein, YK Ho |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
MacDonald IM, et al. A practical diagnostic test for choroideremia. Ophthalmology 105: 1637-1640, 1998. PubMed: 9754170
Andres DA, et al. Mutational analysis of alpha-subunit of protein farnesyltransferase. Evidence for a catalytic role. J. Biol. Chem. 268: 1383-1390, 1993. PubMed: 8419339
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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