| 產(chǎn)品名稱 |
I/24.D6 |
| 商品貨號(hào) |
B164816 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an epitope encoded by variable exon C of mouse CD45. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse CD45RC |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse CD45RC |
| Comments |
Animals were immunized with activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an epitope encoded by variable exon C of mouse CD45. This epitope is expressed at high levels on essentially all mouse B cells and at lower levels on virtually all CD8 positive (CD8+) splenic T cells. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
1% non-essential amino acids and 50 uM 2-mercaptoethanol, 80%; ORIGEN
(Hybridoma Cloning Factor), 10%; heat-inactivated fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgM |
| Name of Depositor |
G Lazlo, KS Hathcock |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Hathcock KS, et al. Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. J. Immunol. 148: 19-28, 1992. PubMed: 1370168
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