| 產品名稱 | Hs 742.Sk |
|---|---|
| 商品貨號 | B164732 |
| Organism | Homo sapiens, human |
| Tissue | skin |
| Product Format | flask |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 71 years |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 47; range = 44 to 47 |
| Images |  |
| Derivation | The line was established from apparently normal tissue from a patient who had adenocarcinoma of the breast (see ATCC CRL-7482). |
| Comments | Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X CSF1PO: 10,12 D13S317: 9,12 D16S539: 11,12 D5S818: 12 D7S820: 8,9 THO1: 6,9.3 TPOX: 8 vWA: 17,19 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
