| Applications |
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria. Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation. |
| Comments |
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells. Since intracellular Listeria escape into the cytoplasm where they replicate, the cell line is a useful tool in the study of the permissive intracellular growth cycle of Listeria. Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation. |
| References |
Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.
Drevets DA, Elliott AM. Fluorescence labeling of bacteria for studies of intracellular pathogenesis. J. Immunol. Methods 187: 69-79, 1995. PubMed: 7490459
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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