| 產(chǎn)品名稱 |
GC-1 spg |
| 商品貨號(hào) |
B164496 |
| Organism |
Mus musculus, transgenic for SV40 early region, mouse, transgenic for SV40 early region |
| Tissue |
testis |
| Cell Type |
transformed with pSV3-neo |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain SV-40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
spermatogonia |
| Age |
10 days |
| Gender |
male |
| Strain |
BALB/c |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance). |
| Clinical Data |
male |
| Comments |
The line shows characteristics of a stage between type B spermatogonia and primary spermatocytes.
The cells express two testis specific isoproteins, cytochrome c and lactate dehydrogenase C4.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
MC Hofmann |
| Deposited As |
mouse, transgenic for SV40 early region |
| References |
Hofmann MC, et al. Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201: 417-435, 1992. PubMed: 1322317
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