| 產品名稱 |
F18 AF1 |
| 商品貨號 |
B164423 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified CSF-I. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with purified CSF-I. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420). |
| Complete Growth Medium |
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Twice per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Amgen, Inc. |
| U.S. Patent Number |
|
| References |
Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985
. . Hybridoma 2: 137, 1983.
|
