| 產品名稱 |
EOMA-GFP |
| 商品貨號 |
B164414 |
| Organism |
Mus musculus, mouse |
| Cell Type |
endothelial |
| Product Format |
frozen |
| Morphology |
endothelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain SV40 and HSV viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hemangioendothelioma |
| Age |
adult |
| Applications |
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1). The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. |
| Comments |
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1). The vector contains cytomegalovirus (CMV), SV40 and Herpes simplex virus (HSV) viral DNA sequences and the neomycin resistance gene. The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. For additional details see EOMA (ATCC CRL-2586). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
0.2 mg/ml G -418
fetal bovine serum to a final concentration of 10%
|
| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
R Auerbach |
| Deposited As |
mouse |
| Year of Origin |
1998 |
| References |
Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185
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