| 產(chǎn)品名稱 |
EH17a |
| 商品貨號(hào) |
B164396 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with human, bovine and rat annexin I. |
| Derivation |
Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human annexin I |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human annexin I |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with human, bovine and rat annexin I. It does not inhibit annexin I mediated phospholipid vesicle aggregation. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
JD Ernst |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ernst JD, et al. Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats. J. Biol. Chem. 266: 6670-6673, 1991. PubMed: 1707872
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