| Applications |
DT95 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken. After one transfer in vivo, the DT95 cell line was established. The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA. The cell line exhibits a more differentiated plasmacytoid phenotype than DT40 (see ATCC CRL-2111). Infectivity assays revealed that DT95 does not release infectious RAV-1. |
| Comments |
DT95 is an avian leukosis virus (ALV) induced bursal lymphoma cell line derived from a Hyline SC chicken. The original lymphoma was induced by viral infection of a 1 day old chicken with Rous associated virus 1 (RAV-1). Cell suspensions prepared from tumors that developed within the bursa of Fabricus were transferred intravenously into young syngeneic recipient chickens. After one transfer in vivo, the DT95 cell line was established. The cell line contains proviral DNA sequences integrated upstream from the c-myc proto-oncogene and expresses increased levels of c-myc RNA. In vitro, both the c-rel gene and the v-rel oncogenes induce major histocompatibility (MHC) class I, MHC class II, and interleukin-2 receptor (IL-2R) antigens on the DT95 cell line. The expression of MHC class II is induced more rapidly by v-rel than c-rel and several weeks after infection, rel induced class II antigen as much as 50 fold more efficiently than c-rel. The cell line exhibits a more differentiated plasmacytoid phenotype than DT40 (see ATCC CRL-2111). Infectivity assays revealed that DT95 does not release infectious RAV-1. |
| References |
Hrdlickova R, et al. v-rel induces expression of three avian immunoregulatory surface receptors more efficiently than c-rel. J. Virol. 68: 308-319, 1994. PubMed: 8254742
Humphries EH, Zhang G. V-rel and C-rel modulate the expression of both bursal and non-bursal antigens on avian B-cell lymphomas. Curr. Top. Microbiol. Immunol. 182: 475-483, 1992. PubMed: 1490388
Baba TW, et al. Cell lines derived from avian lymphomas exhibit two distinct phenotypes. Virology 144: 139-151, 1985. PubMed: 2998040
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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