| 產(chǎn)品名稱(chēng) |
Detroit 551 |
| 商品貨號(hào) |
B164353 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
fetus |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
normal human female; diploid. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Clinical Data |
fetus Caucasian female |
| Virus Susceptibility |
SV40 virus
Human poliovirus 1
Herpes simplex virus
Vesicular stomatitis virus
|
| Comments |
The cells have a finite lifespan of about 25 serial passages from the tissue of origin.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
This cell line can be transformed by SV40. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,12 D13S317: 11,12 D16S539: 12,13 D5S818: 12,13 D7S820: 10,12 THO1: 9,9.3 TPOX: 8 vWA: 18 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
CS Stulberg |
| Deposited As |
Homo sapiens |
| Year of Origin |
1965 |
| References |
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
Miyatake S, et al. Transcriptional targeting of herpes simplex virus for cell-specific replication. J. Virol. 71: 5124-5132, 1997. PubMed: 9188579
|
