| Applications |
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357). Both cell lines produce granulocyte colony stimulating factor (G-CSF). When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections. The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection. Neither the lymphokine or cell line induces viral pathogenesis in chickens. For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours. |
| Comments |
Animals were immunized with Salmonella enteritidis. Spleens were harvested and T cells were isolated. T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770). Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357). Both cell lines produce granulocyte colony stimulating factor (G-CSF). When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections. The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection. Neither the lymphokine or cell line induces viral pathogenesis in chickens. For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours. |
| References |
Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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