| Comments |
This transformed cell line was derived from B-cells of a patient undergoing surgical resection of colorectal cancer. The patient was actively immunized with autologous tumor antigen. Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. Co88BV59H21-2V67-66 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1. Both the 88BV59 antibody and the 16-88 antibody recognize the same tumor associated antigen, but react with different epitopes on that antigen. The 16-88 antibody (LiCO 16-88) is available as ATCC HB-8495. The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538). Co88BV59H21-2 was then exposed to EBV under conditions suitable for transformation to produce Co88BV59H21-2V67-66 (ATCC CRL-11539). |
| References |
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