| 產品名稱 |
CKMM 14.15 |
| 商品貨號 |
B164261 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen 1.0 mL |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to both CK-MM and CK-MB. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CK-MM and CK-MB (creatine kinase - MM and MB) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CK-MM and CK-MB (creatine kinase - MM and MB) |
| Comments |
Animals were immunized with human creatine kinase. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to both CK-MM and CK-MB. Binding of the antibody inhibits the enzyme activity. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM
L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate,
4.5 g/L glucose and 1.0 mM sodium pyruvate, 95%; fetal bovine
serum, 5%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Volume |
1.0 mL |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
Eastman-Kodak Co. |
| Deposited As |
rat (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Daiss JL. Blend of monoclonal antibodies. US Patent 4,950,592 dated Aug 21 1990
|
