| 產品名稱 |
CDR1 |
| 商品貨號 |
B164221 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen |
| Comments |
Animals were immunized with mouse thymic stromal tissue. Spleen cells were fused with Sp2/0 myeloma cells. The antibody does not react with the thymic medulla, and the reactivity appears to be identical to that reported for the 6C3 hybridoma (Proc. Natl. Acad. Sci. USA 81:4434-4438, 1984). It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Antigenic Determinant |
Abelson murine leukemia virus, 16-kDa antigen |
| Name of Depositor |
RV Rouse |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Pillemer E, et al. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc. Natl. Acad. Sci. USA 81: 4434-4438, 1984. PubMed: 6611551
Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413
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