| 產(chǎn)品名稱 |
CCD-42Sk |
| 商品貨號(hào) |
B164211 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
4 years |
| Gender |
male |
| Applications |
The line was derived from normal skin of a child that died of multiple congenital defects. |
| Derivation |
The line was derived from normal skin of a child that died of multiple congenital defects. |
| Clinical Data |
The line was derived from normal skin of a child that died of multiple congenital defects. male |
| Comments |
The line was derived from normal skin of a child that died of multiple congenital defects. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,13 D16S539: 9,11 D5S818: 12,13 D7S820: 11 THO1: 7,8 TPOX: 8,11 vWA: 15 |
