| 產品名稱 |
CCD-1138Sk |
| 商品貨號 |
B164192 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
43 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
The cell line was derived from normal skin taken from the abdomen. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The cell line was derived from normal skin taken from the abdomen. |
| Clinical Data |
male Caucasian 43 years |
| Comments |
The cell line was derived from normal skin taken from the abdomen. Cells senesce after approximately 25 population doublings. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
P.Wood, ATCC |
| Deposited As |
human |
| References |
Cells from the current passage (2) have experienced approximately 4.5 population doublings beyond the biopsy material. The cells are capable of at least 13 additional population doublings before the onset of senescence.
skin taken from the abdomen
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