| 產(chǎn)品名稱 |
CC20 |
| 商品貨號 |
B164128 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the bovine CD1 antigen expressed on immature cortical thymocytes. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine CD1 (BoCD1w2) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine CD1 (BoCD1w2) |
| Comments |
Animals were immunized with bovine thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for the bovine CD1 antigen expressed on immature cortical thymocytes. It is homologous to antibodies in the human CD1b cluster. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG2a |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546
. . Vet. Immunol. Immunopathol. 32: 1993..
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