| 產品名稱 |
BE2 |
| 商品貨號 |
B164020 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against glycosphingolipid having type II chain H structure |
| Cellular Products |
immunoglobulin; monoclonal antibody; against glycosphingolipid having type II chain H structure |
| Comments |
Animals were immunized with naked Salmonella minnesota to which had been adsorbed a mixture of neutral glycolipids from human type O erythrocytes. Spleen cells were fused with NS-1 myeloma cells. At 37C, this antibody agglutinates type O and A2 but not type B or A1 erythrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
WW Young |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743
|
