Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells).
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells
Cellular Products
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells
Comments
Animals were immunized with KG-1a cells (see ATCC CCL-246.1).
Spleen cells were fused with myeloma cells.
The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells).
Complete Growth Medium
RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
