| 產品名稱 |
AE-1 |
| 商品貨號 |
B163909 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to human and monkey acetylcholinesterase, but reacts with a different epitope than AE-2 (ATCC HB-73). Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with purified human erythrocyte acetylcholinesterase. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human acetylcholinesterase |
| Comments |
Animals were immunized with purified human erythrocyte acetylcholinesterase. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to human and monkey acetylcholinesterase, but reacts with a different epitope than AE-2 (ATCC HB-73). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. An inoculum of 2 X 10(5) viable cells/ml is recommended.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
IgG1 |
| Antigenic Determinant |
Acetylcholin esterase, human |
| Name of Depositor |
DM Fambrough |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 79: 1078-1082, 1982. PubMed: 6175961
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