| 產(chǎn)品名稱 |
Ad Hot |
| 商品貨號(hào) |
B163908 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Ehlers-Danlos syndrome, type II |
| Age |
53 years |
| Gender |
female |
| Clinical Data |
The tissue donor suffered from type II Ehlers-Danlos syndrome, and was the mother of Ray Hot (ATCC CRL-1226). female |
| Comments |
The tissue donor suffered from type II Ehlers-Danlos syndrome, and was the mother of Ray Hot (ATCC CRL-1226). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |
