| 產品名稱 |
AB1-2 |
| 商品貨號 |
B163902 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
lymph node |
| Cell Type |
hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
A/J |
| Applications |
The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against T15 idiotype |
| Cellular Products |
immunoglobulin; monoclonal antibody; against T15 idiotype |
| Comments |
Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas. The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
mouse IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor |
JF Kearney |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186
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